Increase and decrease in protein phosphorylation by tumor promoter 12-0-tetradecanoylphorbol-13-acetate in HL-60 cells.
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چکیده
منابع مشابه
Tumor promoter 12-O-tetradecanoylphorbol-13-acetate and sn-1,2-dioctanoylglycerol increase the phosphorylation of protein kinase C in cells.
Phosphorylation of protein kinase C (PKC) may be an important mode of regulation of this enzyme that plays a key role in mouse skin tumor promotion and in mammalian cell signal transduction. To investigate this possibility, PKC was specifically immunoprecipitated from Abelson murine leukemia virus-transformed normal rat kidney cells that had been metabolically labeled with [32P]orthophosphoric ...
متن کاملInhibition by palmitoylcarnitine of adhesion and morphological changes in HL-60 cells induced by 12-O-tetradecanoylphorbol-13-acetate.
Effects of DL-palmitoylcarnitine (PC), an inhibitor of calciumactivated, phospholipid-dependent protein kinase (protein kinase C), on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell differentiation were investigated in human promyelocytic leukemia cells (HL-60). TPA caused HL-60 cell adhesion concomitant with morphological changes, and an increase in acid phosphatase activity. The media...
متن کاملDivergent responses in epidermal basal cells exposed to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate.
Mouse epidermal basal cells can be selectively cultivated in medium with 0.02 to 0.09 mM Ca2+ and can be induced to differentiate by medium containing 1.2 mM Ca2+. Basal cell cultures were studied to determine if all cells in this population responded identically to the skin tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Studies on the induction of the enzyme epidermal transglutamin...
متن کاملInsulinotropic effect of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate in rat pancreatic islets.
In isolated rat pancreatic islets, the tumor-promoting agent 12-O-tetradecanoylphorbol-13-acetate (TPA), when used in the 2.10(-9) to 2.10(-7) M range, was found to stimulate insulin release both in the absence and presence of glucose. The non-tumor-promoting agent 4-methylphorbol-12,13-didecanoate failed to stimulate insulin release. The insulinotropic capacity of TPA was enhanced by glucose i...
متن کاملRegulation of Release of Hepatocyte Growth Factor From Human Promyelocytic Leukemia Cells, HL-60, by 1,25-Dihydroxyvitamin D,, 12-0-Tetradecanoylphorbol 13-Acetate, and Dibutyryl Cyclic Adenosine Monophosphate
Hepatocyte growth factor (HGF) secreted from human promyelocytic leukemia cell line, HL-60, is indistinguishable from HGF in human plasma and its release is significantly stimulated by 1 2-0-tetradecanoylphorbol-I 3-acetate (TPA), a differentiation-inducer of HL-60 cells into monocytes/macrophages (Nishino T et al: Biochem Biophys Res Commun 181 :323, 1991). TPA stimulated HGF release from the ...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1985
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)63402-5